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celltrace yellow proliferation kit (#c34567)  (Thermo Fisher)


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    Thermo Fisher celltrace yellow proliferation kit (#c34567)
    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with <t>CellTrace</t> yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. <t>Proliferation</t> was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
    Celltrace Yellow Proliferation Kit (#C34567), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+yellow+proliferation+kit+c34567/pmc11501018-52-0-8
    Average 90 stars, based on 1 article reviews
    celltrace yellow proliferation kit (#c34567) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells"

    Article Title: Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012601

    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with CellTrace yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. Proliferation was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
    Figure Legend Snippet: A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with CellTrace yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. Proliferation was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).

    Techniques Used: Cell Culture, Flow Cytometry, Infection, Staining

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    Article Snippet: .. Following counting, T cells were stained with CellTrace Yellow Proliferation kit (Thermo Fisher Scientific, C34567). ..



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    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with <t>CellTrace</t> yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. <t>Proliferation</t> was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
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    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with <t>CellTrace</t> yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. <t>Proliferation</t> was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
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    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with <t>CellTrace</t> yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. <t>Proliferation</t> was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
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    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with <t>CellTrace</t> yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. <t>Proliferation</t> was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).
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    Image Search Results


    A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with CellTrace yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. Proliferation was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).

    Journal: PLOS Pathogens

    Article Title: Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells

    doi: 10.1371/journal.ppat.1012601

    Figure Lengend Snippet: A: Workflow to identify 5 FDA-approved compounds with transcriptional profiles similar to lead LRA using CMap analysis. Figure created with BioRender. B: CD69 MFI of matched cultured T CM (n = 9) were measured by flow cytometry after treatment with 30 IU/mL IL-2 or IL-2 plus 100 μ M HODHBt or 10 μ M of indicated compounds for 72 hours. Significant p values over cells treated with IL-2 alone. C: Reactivation of latent HIV in T CM (n = 7) measured by flow cytometry after treatment with 100 μ M HODHBt or 10 μ M Isotretinoin alone, plus 100 ng/mL IL-15, or α CD3/CD28. D: Calculation of synergy for LRA combinations using the Bliss independence model (n = 7). Data is presented as the difference between the observed and expected fractional response. E: Viability of uninfected or latently infected matched conditions (n = 7). F: Memory CD4 T cells were stained with CellTrace yellow and treated with 100 μ M HODHBt or 10 μ M Isotretinoin alone or in combination with 100 ng/mL IL-15, or α CD3/CD28 for 7 days. Proliferation was measured by flow cytometry (n = 5). Wilcoxon matched-pairs signed rank test was used to calculate p values (*p < 0.05; **p < 0.01).

    Article Snippet: CellTrace Yellow proliferation kit (#C34567) was purchased from Invitrogen.

    Techniques: Cell Culture, Flow Cytometry, Infection, Staining